Polyhydroxybutyrate Production by Heterotrophic Method Assisted by Nanotechnology
Keywords:
Ultrasound, Bioplastic, Polyhydroxybutyrate, Piezoelectric, Fructose, Ralstonia EutrophaAbstract
Bioplastics have recently garnered widespread attention as an environmentally friendly alternative in the polymer industry. The production of traditional plastics from petrochemical sources accounts for 12% of global oil consumption and generates non-biodegradable waste, leading to adverse environmental impacts. Consequently, bioplastics such as polyhydroxyalkanoates (PHAs), particularly polyhydroxybutyrate (PHB), have gained prominence as biodegradable and eco-friendly substitutes. In this study, piezoelectric ZnO nanoparticles were synthesized via the hydrothermal method and applied using ultrasound to enhance PHB production by Ralstonia eutropha. Results indicated that at a ZnO concentration of 0.5 gr/lit, PHB production reached under ultrasound in heterotrophic culture, which was 1.4 times higher than the control group. This research demonstrates ZnO's efficiency, stability, and biocompatibility in a hybrid heterotroPHic-piezoelectric system, proposing it as a promising approach for bioplastic production and CO2 reduction in line with green chemistry principles. For large-scale industrial production, further investigation is required to optimize culture conditions and elucidate the exact electron transfer mechanism between the catalyst and bacteria.
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